Indirect enzymatic phosphorylation: preparation of dihydroxyacetone phosphate

Abstract

Starting from natural phosphatidyl choline, the substitution of the choline polar head for an alcohol donor is catalysed by phospholipase D; subsequent hydrolysis of the new phospholipid obtained in the presence of phospholipase C allows the obtainment of the phosphate of the alcohol donor; the overall process is the net transfer of the phosphate moiety from a phospholipid to a primary alcohol and the procedure is verified in the preparation of dihydroxyacetone phosphate