Issue 81, 2014

A cleavable azide resin for direct click chemistry mediated enrichment of alkyne-labeled proteins

Abstract

The direct conjugation of a labeled proteome to a cleavable azide resin utilizing the copper-catalyzed azide alkyne cycloaddition is demonstrated. The procedure omits the classical streptavidin- and biotin-based affinity enrichment step and represents an operationally simpler, cheaper and less contaminated alternative for protein purification.

Graphical abstract: A cleavable azide resin for direct click chemistry mediated enrichment of alkyne-labeled proteins

Supplementary files

Article information

Article type
Communication
Submitted
08 Jul 2014
Accepted
21 Aug 2014
First published
21 Aug 2014

Chem. Commun., 2014,50, 12098-12100

Author version available

A cleavable azide resin for direct click chemistry mediated enrichment of alkyne-labeled proteins

C. Sibbersen, L. Lykke, N. Gregersen, K. A. Jørgensen and M. Johannsen, Chem. Commun., 2014, 50, 12098 DOI: 10.1039/C4CC05246C

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