Issue 15, 2015
From the journal:

Chemical Communications

Smart nanoprobes for the detection of alkaline phosphatase activity during osteoblast differentiation†

Eun-Kyung Lim,ab   Joo Oak Keem,b   Hui-suk Yun,c   Jinyoung Jung*abd  and  Bong Hyun Chung*abd  

* Corresponding authors

a BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea
E-mail: jyjeong@kribb.re.kr, chungbh@kribb.re.kr
Fax: +82-42-879-8594
Tel: +82-42-879-8456

b BioNano Health Guard Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea

c Powder & Ceramics Division, Korea Institute of Materials Science (KIMS), 797 Changwondaero, Seongsangu, Changwon 642-831, Republic of Korea

d Nanobiotechnology Major, School of Engineering, University of Science and Technology (UST), 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea

Abstract

Gold nanoparticle-conjugated fluorescent hydroxyapatite (AuFHAp) was developed as a smart nanoprobe for measuring alkaline phosphatase (ALP) activity. AuFHAp showed NIR fluorescence due to the hydrolysis of its phosphate groups by ALP. In addition, gold nanoparticles help reduce the nonspecific signal by absorbing nonspecific fluorescence. Through in vitro tests, we confirmed that the AuFHAp probe was capable of detecting ALP levels related to osteoblast activity in living cells with high fluorescence intensity.

Graphical abstract: Smart nanoprobes for the detection of alkaline phosphatase activity during osteoblast differentiation

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Article information

DOI
https://doi.org/10.1039/C4CC09620G
Article type
Communication
Submitted
02 Dec 2014
Accepted
17 Jan 2015
First published
19 Jan 2015

Chem. Commun., 2015,51, 3270-3272

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Smart nanoprobes for the detection of alkaline phosphatase activity during osteoblast differentiation

E. Lim, J. O. Keem, H. Yun, J. Jung and B. H. Chung, Chem. Commun., 2015, 51, 3270 DOI: 10.1039/C4CC09620G

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