Issue 10, 2016

Estimation of methylglyoxal in cow milk – an accurate electrochemical response time based approach

Abstract

Cow milk contains carbohydrates that are prone to produce methylglyoxal (MG) which plays a major role in chronic complications of diabetes. In this context, an electrochemical biosensor based on a glyoxalase 1 (GLO 1) modified platinum electrode (Pt) with a ceria nano-interface (CeO2) was developed. The fabricated Pt/CeO2/GLO 1/chitosan nano-bioelectrode reduced MG and hemithioacetal at −0.771 and −0.558 V respectively in the presence of glutathione (GSH). Michaelis–Menten and Hill models were employed for the estimation of the response time. The accuracy of these models was validated by calculating the relative prediction error. The modified Hill model showed the best results in validation. The sensitivity of the Pt/CeO2/GLO 1/chitosan nano-bioelectrode at −0.771 V was 2.868 μA μM−1 over a linear range between 5 and 50 μM, with a detection limit of 2.14 nM, a quantification limit of 7.12 nM and a response time of less than 39 s. The fabricated electrode was demonstrated to be highly reproducible with a relative standard deviation of 1.02% over 10 successive amperometric calibrations. It also showed good recovery (99.21–101.72%), thus providing a promising tool for the analysis of MG in cow milk.

Graphical abstract: Estimation of methylglyoxal in cow milk – an accurate electrochemical response time based approach

Article information

Article type
Paper
Submitted
03 Nov 2015
Accepted
10 Feb 2016
First published
12 Feb 2016

Anal. Methods, 2016,8, 2207-2217

Author version available

Estimation of methylglyoxal in cow milk – an accurate electrochemical response time based approach

B. L. Ramachandra, S. Vedantham, U. M. Krishnan, N. Nesakumar and J. B. Balaguru Rayappan, Anal. Methods, 2016, 8, 2207 DOI: 10.1039/C5AY02901E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements