Issue 78, 2015
From the journal:

Chemical Communications

A novel electrochemical immunosensor for the quantitative detection of 5-hydroxymethylcytosine in genomic DNA of breast cancer tissue†

Zhiqing Yang,‡a   Wenjing Jiang,‡a   Fei Liu,b   Yunlei Zhou,a   Huanshun Yin*a  and  Shiyun Ai*a  

* Corresponding authors

a College of Chemistry and Material Science, Shandong Agricultural University, Taian, Shandong, P. R. China
E-mail: yinhs@sdau.edu.cn, ashy@sdau.edu.cn
Fax: +86-538-8242251

b The Tumor Center of Taian City Central Hospital, Taian, Shandong, P. R. China

Abstract

A novel electrochemical immunosensor was fabricated for the quantitative detection of 5-hydroxymethylcytosine (5-hmC) in genomic DNA based on anti-5-hmC antibody, biotin functionalized phos-tag and avidin functionalized alkaline phosphatase. It is demonstrated that the levels of 5-hmC are dramatically reduced in human breast cancer tissue compared with those in normal tissue.

Graphical abstract: A novel electrochemical immunosensor for the quantitative detection of 5-hydroxymethylcytosine in genomic DNA of breast cancer tissue

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C5CC05921F
Article type
Communication
Submitted
16 Jul 2015
Accepted
12 Aug 2015
First published
12 Aug 2015

Chem. Commun., 2015,51, 14671-14673

Author version available

Download author version (PDF)

Permissions

Request permissions

A novel electrochemical immunosensor for the quantitative detection of 5-hydroxymethylcytosine in genomic DNA of breast cancer tissue

Z. Yang, W. Jiang, F. Liu, Y. Zhou, H. Yin and S. Ai, Chem. Commun., 2015, 51, 14671 DOI: 10.1039/C5CC05921F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements