Issue 8, 2016

Stimulated Raman scattering microscopy: an emerging tool for drug discovery

Abstract

Optical microscopy techniques have emerged as a cornerstone of biomedical research, capable of probing the cellular functions of a vast range of substrates, whilst being minimally invasive to the cells or tissues of interest. Incorporating biological imaging into the early stages of the drug discovery process can provide invaluable information about drug activity within complex disease models. Spontaneous Raman spectroscopy has been widely used as a platform for the study of cells and their components based on chemical composition; but slow acquisition rates, poor resolution and a lack of sensitivity have hampered further development. A new generation of stimulated Raman techniques is emerging which allows the imaging of cells, tissues and organisms at faster acquisition speeds, and with greater resolution and sensitivity than previously possible. This review focuses on the development of stimulated Raman scattering (SRS), and covers the use of bioorthogonal tags to enhance sample detection, and recent applications of both spontaneous Raman and SRS as novel imaging platforms to facilitate the drug discovery process.

Graphical abstract: Stimulated Raman scattering microscopy: an emerging tool for drug discovery

Article information

Article type
Review Article
Submitted
07 Sep 2015
First published
03 Feb 2016
This article is Open Access
Creative Commons BY license

Chem. Soc. Rev., 2016,45, 2075-2089

Author version available

Stimulated Raman scattering microscopy: an emerging tool for drug discovery

W. J. Tipping, M. Lee, A. Serrels, V. G. Brunton and A. N. Hulme, Chem. Soc. Rev., 2016, 45, 2075 DOI: 10.1039/C5CS00693G

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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