Photolysis quantum yield measurements in the near-UV; a critical analysis of 1-(2-nitrophenyl)ethyl photochemistry

Abstract

The photolysis quantum yield, Q_p, of 1-(2-nitrophenyl)ethyl phosphate (caged P_i) measured in the near-UV (342 nm peak with 60 nm half-bandwidth) is 0.53 and is based on results reported in 1978 (Biochemistry, 17, 1929–1935). This article amplifies methodology for determining that Q_p in view of different recent estimates. Some general principles together with other examples relating to measurement of Q_p values are discussed together with their relevance to biological research.