Issue 10, 2016

Carbon monoxide attenuates the effects of snake venoms containing metalloproteinases with fibrinogenase or thrombin-like activity on plasmatic coagulation

Abstract

Exposure of plasma to iron and carbon monoxide (CO) renders fibrinogen resistant to fibrinogenolytic or thrombin-like activity contained in pit viper venom. However, the direct effects of iron/CO on venom activity are unknown. Thus, we assessed if four different, metalloproteinase containing snake venoms exposed to iron/CO or CO alone could attenuate their fibrinogenolytic or thrombin-like activity. Venom (0–500 μg ml−1) was exposed to 0–10 μM FeCl3 and/or 0–100 μM carbon monoxide releasing molecule-2 (CORM-2), or inactivated CORM-2 (iCORM-2) for 3 min at room temperature. Venom solution (0–8 μg ml−1 final concentration) was then placed in citrated human plasma containing tissue factor, followed by CaCl2 addition for commencement of coagulation. Data were determined with thrombelastography for 10–15 min at 37 °C. Iron had no effect on the first venom tested, so only CO was investigated subsequently. Exposure of venom to CO attenuated fibrinogenolytic or thrombin-like activity, and iCORM-2 did not affect the venom activities. Further investigation of the effect of CO exposure on similar venoms is justified.

Graphical abstract: Carbon monoxide attenuates the effects of snake venoms containing metalloproteinases with fibrinogenase or thrombin-like activity on plasmatic coagulation

Article information

Article type
Research Article
Submitted
15 Jun 2016
Accepted
26 Jul 2016
First published
29 Jul 2016
This article is Open Access
Creative Commons BY license

Med. Chem. Commun., 2016,7, 1973-1979

Carbon monoxide attenuates the effects of snake venoms containing metalloproteinases with fibrinogenase or thrombin-like activity on plasmatic coagulation

V. G. Nielsen and C. M. Bazzell, Med. Chem. Commun., 2016, 7, 1973 DOI: 10.1039/C6MD00336B

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