Issue 87, 2017
From the journal:

Chemical Communications

Temperature-responsive split aptamers coupled with polymerase chain reaction for label-free and sensitive detection of cancer cells†

Jinlu Tang,a   Xiaoxiao He,a   Yanli Lei,a   Hui Shi,*a   Qiuping Guo,a   Jianbo Liu,a   Dinggeng He,a   Lv’an Yana  and  Kemin Wang ORCID logo *a  

* Corresponding authors

a State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha, Hunan 410082, China
E-mail: kmwang@hnu.edu.cn, huishi_2009@hnu.edu.cn
Fax: +86-731-88821566
Tel: +86-731-88821566

Abstract

A label-free and general thermo-controlled split apta-PCR strategy was first developed for the sensitive and specific detection of cancer cells. By integrating the temperature-responsive function of split aptamers with PCR amplification, a facile fluorescence assay of liver cancer SMMC-7721 cells was successfully realized with the detection of as low as 100 cells.

Graphical abstract: Temperature-responsive split aptamers coupled with polymerase chain reaction for label-free and sensitive detection of cancer cells

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Article information

DOI
https://doi.org/10.1039/C7CC06218D
Article type
Communication
Submitted
09 Aug 2017
Accepted
10 Oct 2017
First published
10 Oct 2017

Chem. Commun., 2017,53, 11889-11892

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Temperature-responsive split aptamers coupled with polymerase chain reaction for label-free and sensitive detection of cancer cells

J. Tang, X. He, Y. Lei, H. Shi, Q. Guo, J. Liu, D. He, L. Yan and K. Wang, Chem. Commun., 2017, 53, 11889 DOI: 10.1039/C7CC06218D

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