Issue 98, 2018

A triarylphosphine–trimethylpiperidine reagent for the one-step derivatization and enrichment of protein post-translational modifications and identification by mass spectrometry

Abstract

We report a new reagent that is capable of both chemical derivatization and selective enrichment of azide-labeled PTM peptides for sensitive identification by mass spectrometry (MS). Facile sample recovery, enhanced ionization and fragmentation in MS of the enriched PTM peptides are achieved, which leads to the identification of 3293 O-GlcNAc peptides and the location of 1706 sites in HeLa cells and efficiently expands the current mapping scale.

Graphical abstract: A triarylphosphine–trimethylpiperidine reagent for the one-step derivatization and enrichment of protein post-translational modifications and identification by mass spectrometry

Supplementary files

Article information

Article type
Communication
Submitted
21 Oct 2018
Accepted
23 Oct 2018
First published
02 Nov 2018

Chem. Commun., 2018,54, 13790-13793

A triarylphosphine–trimethylpiperidine reagent for the one-step derivatization and enrichment of protein post-translational modifications and identification by mass spectrometry

B. Huo, W. Zhang, X. Zhao, H. Dong, Y. Yu, J. Wang, X. Qian and W. Qin, Chem. Commun., 2018, 54, 13790 DOI: 10.1039/C8CC08416E

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