Issue 21, 2019
From the journal:

Nanoscale

Packaging of DNA origami in viral capsids†

Idit Kopatz, ORCID logo ‡*a   Ran Zalk, ORCID logo ‡b   Yael Levi-Kalisman,cf   Efrat Zlotkin-Rivkin,d   Gabriel A. Frankbe  and  Stanislav Kler*a  

* Corresponding authors

a Geza Ad Ltd, Givatayim 53251, Israel
E-mail: idit.kopatz@geza-ad.com, slavakler1@gmail.com

b The National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer Sheva, Israel

c The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel

d The Protein Expression Facility, Wolfson Centre for Applied Structural Biology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel

e Department of life sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel

f The Institute for Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel

Abstract

Here we show the encapsulation of 35 nm diameter, nearly-spherical, DNA origami by self-assembly of SV40-like (simian virus 40) particles. The self-assembly of this new type of nanoparticles is highly reproducible and efficient. The structure of these particles was determined by cryo-EM. The capsid forms a regular SV40 lattice of T = 7d icosahedral symmetry and the structural features of encapsulated DNA origami are fully visible. These particles are a promising biomaterial for use in various medical applications.

Graphical abstract: Packaging of DNA origami in viral capsids

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C8NR10113B
Article type
Communication
Submitted
14 Dec 2018
Accepted
11 Apr 2019
First published
17 Apr 2019

Nanoscale, 2019,11, 10160-10166

Permissions

Request permissions

Packaging of DNA origami in viral capsids

I. Kopatz, R. Zalk, Y. Levi-Kalisman, E. Zlotkin-Rivkin, G. A. Frank and S. Kler, Nanoscale, 2019, 11, 10160 DOI: 10.1039/C8NR10113B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements