Issue 47, 2018

On measuring the acoustic state changes in lipid membranes using fluorescent probes

Abstract

Ultrasound is increasingly being used to modulate the properties of biological membranes for applications in drug delivery and neuromodulation. While various studies have investigated the mechanical aspects of the interaction such as acoustic absorption and membrane deformation, it is not clear how these effects transduce into biological functions, for example, changes in the permeability or the enzymatic activity of the membrane. A critical aspect of the activity of an enzyme is the thermal fluctuations of its solvation or hydration shell. Thermal fluctuations are also known to be directly related to membrane permeability. Here solvation shell changes of lipid membranes subject to an acoustic impulse were investigated using a fluorescence probe, Laurdan. Laurdan was embedded in multi-lamellar lipid vesicles in water, which were exposed to broadband pressure impulses of the order of 1 MPa peak amplitude and 10 µs pulse duration. An instrument was developed to monitor changes in the emission spectrum of the dye at two wavelengths with sub-microsecond temporal resolution. The experiments show that changes in the emission spectrum, and hence the fluctuations of the solvation shell, are related to the changes in the thermodynamic state of the membrane and correlated with the compression and rarefaction of the incident sound wave. The results suggest that acoustic fields affect the state of a lipid membrane and therefore can potentially modulate the kinetics of channels and enzymes embedded in the membrane.

Graphical abstract: On measuring the acoustic state changes in lipid membranes using fluorescent probes

Article information

Article type
Paper
Submitted
09 Aug 2018
Accepted
15 Nov 2018
First published
15 Nov 2018

Soft Matter, 2018,14, 9702-9712

On measuring the acoustic state changes in lipid membranes using fluorescent probes

S. Shrivastava, R. O. Cleveland and M. F. Schneider, Soft Matter, 2018, 14, 9702 DOI: 10.1039/C8SM01635F

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