Issue 35, 2019

Developing chemically modified redox-responsive proteins as smart therapeutics

Abstract

The conditional control of protein function in response to the physiological changes of diseased cells is essential to develop smart protein therapeutics. Herein, we report a redox-responsive chemical modification of a protein by conjugating an intracellular glutathione (GSH)-cleavable ligand, NSA, onto a protein residue. We demonstrated that the NSA conjugation of Ribonuclease A (RNase A) enabled the control of the protein function by GSH in an aqueous solution and living cells, with extended applications for targeted cancer therapy using a lipid nanoparticle-based intracellular protein delivery strategy.

Graphical abstract: Developing chemically modified redox-responsive proteins as smart therapeutics

Supplementary files

Article information

Article type
Communication
Submitted
21 Jan 2019
Accepted
07 Apr 2019
First published
08 Apr 2019

Chem. Commun., 2019,55, 5163-5166

Developing chemically modified redox-responsive proteins as smart therapeutics

Q. Tang, J. Wang, Y. Jiang, M. Zhang, J. Chang, Q. Xu, L. Mao and M. Wang, Chem. Commun., 2019, 55, 5163 DOI: 10.1039/C9CC00519F

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