Issue 4, 2020

DNAzyme-powered nucleic acid release from solid supports

Abstract

Here, we demonstrate use of a Mg2+-dependent, site-specific DNA enzyme (DNAzyme) to cleave oligos from polyacrylamide gel beads, which is suitable for use in drop-based assays. We show that cleavage efficiency is improved by use of a tandem-repeat cleavage site. We further demonstrate that DNAzyme-released oligos function as primers in reverse transcription of cell-released mRNA.

Graphical abstract: DNAzyme-powered nucleic acid release from solid supports

Supplementary files

Article information

Article type
Communication
Submitted
04 Oct 2019
Accepted
26 Nov 2019
First published
13 Dec 2019

Chem. Commun., 2020,56, 647-650

Author version available

DNAzyme-powered nucleic acid release from solid supports

T. Cao, Y. Wang, Y. Tao, L. Zhang, Y. Zhou, X. Zhang, J. A. Heyman and D. A. Weitz, Chem. Commun., 2020, 56, 647 DOI: 10.1039/C9CC07790A

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