Issue 6, 2019
From the journal:

Lab on a Chip

Paper-based multiplexed vertical flow assay for point-of-care testing†

Hyou-Arm Joung, ORCID logo a   Zachary S. Ballard, ORCID logo ab   Alice Ma, ORCID logo c   Derek K. Tseng,a   Hailemariam Teshome,d   Spencer Burakowski,e   Omai B. Garner,f   Dino Di Carloeg  and  Aydogan Ozcan ORCID logo *abg  

* Corresponding authors

a Electrical & Computer Engineering Department, University of California, Los Angeles, California 90095, USA
E-mail: ozcan@ucla.edu

b California NanoSystems Institute (CNSI), University of California, Los Angeles, California 90095, USA

c Biochemistry Department, University of California, Los Angeles, California 90095, USA

d Neuroscience Department, University of California, Los Angeles, California 90095, USA

e Mechanical Engineering Department, University of California, Los Angeles, California 90095, USA

f Department of Pathology and Laboratory Medicine, University of California, Los Angeles, California 90095, USA

g Bioengineering Department, University of California, Los Angeles, California 90095, USA

Abstract

We developed a multiplexed point-of-care immunodiagnostic assay for antibody detection in human sera made through the vertical stacking of functional paper layers. In this multiplexed vertical flow immunodiagnostic assay (xVFA), a colorimetric signal is generated by gold nanoparticles captured on a spatially-multiplexed sensing membrane containing specific antigens. The assay is completed in 20 minutes, following which the sensing membrane is imaged by a cost-effective mobile-phone reader. The images are sent to a server, where the results are rapidly analyzed and relayed back to the user. The performance of the assay was evaluated by measuring Lyme-specific antibodies in human sera as model target antibodies. The presented platform is rapid, simple, inexpensive, and allows for simultaneous and quantitative measurement of multiple antibodies and/or antigens making it a suitable point-of-care platform for disease diagnostics.

Graphical abstract: Paper-based multiplexed vertical flow assay for point-of-care testing

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Article information

DOI
https://doi.org/10.1039/C9LC00011A
Article type
Paper
Submitted
03 Jan 2019
Accepted
31 Jan 2019
First published
31 Jan 2019

Lab Chip, 2019,19, 1027-1034

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Paper-based multiplexed vertical flow assay for point-of-care testing

H. Joung, Z. S. Ballard, A. Ma, D. K. Tseng, H. Teshome, S. Burakowski, O. B. Garner, D. Di Carlo and A. Ozcan, Lab Chip, 2019, 19, 1027 DOI: 10.1039/C9LC00011A

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