Issue 22, 2019

A magnetic nanofiber-based zwitterionic hydrophilic material for the selective capture and identification of glycopeptides

Abstract

High-performance affinity materials are highly required in the sample preparation process in mass spectrometry-based glycoproteomics studies. In this research, a novel magnetic nanofiber-based zwitterionic hydrophilic material is prepared for glycopeptide enrichment and identification. The one-dimensional hydroxyapatite nanofiber (HN) acted as the supporting substance for immobilizing both Fe3O4 nanoparticles and Au nanoparticles, following the surface modification with a zwitterionic tripeptide L-glutathione (GSH) via the affinity interactions between the thiol group in GSH and both Au and Fe3O4 to form the magHN/Au-GSH nanofiber. Owing to the unique structural features, excellent hydrophilicity, abundant zwitterionic molecules, and strong magnetic responsiveness, the as-prepared magHN/Au-GSH nanofiber possesses satisfactory specificity for glycopeptide enrichment. As a result, the magHN/Au-GSH nanofiber demonstrated great detection sensitivity (2 fmol), satisfying enrichment recovery (89.65%), large binding capacity (100 mg g−1), and high enrichment selectivity (1 : 100) toward glycopeptides. Furthermore, 246 N-glycosylated peptides corresponding to 104 N-glycosylated proteins were identified from only 1 μL human serum, revealing the great potential of this affinity nanofiber for glycopeptide enrichment and glycoproteomics research.

Graphical abstract: A magnetic nanofiber-based zwitterionic hydrophilic material for the selective capture and identification of glycopeptides

Supplementary files

Article information

Article type
Paper
Submitted
16 Feb 2019
Accepted
03 May 2019
First published
07 May 2019

Nanoscale, 2019,11, 10952-10960

A magnetic nanofiber-based zwitterionic hydrophilic material for the selective capture and identification of glycopeptides

W. Huan, J. Zhang, H. Qin, F. Huan, B. Wang, M. Wu and J. Li, Nanoscale, 2019, 11, 10952 DOI: 10.1039/C9NR01441A

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