Issue 45, 2019

The interactions of human ovarian cancer cells and nanotextured surfaces: cell attachment, viability and apoptosis studies

Abstract

Understanding cell responses to the topography they are interacting with has a key role in designing surfaces due to the distinctiveness in the responses of different cell types. Thus far, a variety of surface textures have been fabricated, and the cellular responses of diversified cell lines to the surface textures have been assessed together with surface chemistry. However, the results reported in the literature are contradictory, and also not in-depth for inferring the relevance between cells, surface chemistry, and surface topography. Starting from this point of view, we focused on fabricating surfaces having extracellular matrix-like surface patterns and investigated the influence of patterning on human ovarian cancer cells. In this study, hemispherical protrusion-shaped, nanotextured surfaces were prepared via colloidal lithography and polymer casting methods using monolayer templates prepared from 280 nm, 210 nm, and 99 nm polystyrene particles and polydimethylsiloxane moulds. Then, the surface textures were transferred to biocompatible polycaprolactone films. After the characterisation of the surfaces via atomic force microscopy, X-ray photoelectron spectroscopy, and contact angle measurements, the cellular response to topography was evaluated by cell attachment, viability, and apoptosis studies. The results were compared with non-textured surfaces and control plate wells. The results showed that human ovarian cancer cell attachment increased with nanotexturing, which suggests that nanotexturing may be a promising approach for cancer cell modulation, and may have the potential to introduce new strategies for cancer treatment.

Graphical abstract: The interactions of human ovarian cancer cells and nanotextured surfaces: cell attachment, viability and apoptosis studies

Supplementary files

Article information

Article type
Paper
Submitted
20 May 2019
Accepted
05 Aug 2019
First published
19 Aug 2019
This article is Open Access
Creative Commons BY license

RSC Adv., 2019,9, 25957-25966

The interactions of human ovarian cancer cells and nanotextured surfaces: cell attachment, viability and apoptosis studies

G. Yaşayan, O. Orun, P. Mega Tiber, V. Rožman and S. Koçyiğit Sevinç, RSC Adv., 2019, 9, 25957 DOI: 10.1039/C9RA03783G

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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