Issue 9, 2022

Establishing a quantitative fluorescence assay for the rapid detection of kynurenine in urine

Abstract

The kynurenine metabolite is associated with many diseases and disorders, ranging from diabetes and sepsis to more recently COVID-19. Here we report a fluorescence-based assay for the detection of kynurenine in urine using a specific chemosensor, 3-formyl-4-(ethylthio)-7-(diethylamino)-coumarin. The assay produces a linear response at clinically relevant ranges (1–20 μM), with a limit of detection of 0.7 μM. The average standard addition recoveries of kynurenine in synthetic urine samples are near to 100%, and the relative standard deviation values are less than 8%. The established fluorescence assay for quantitative analysis of kynurenine in urine is facile, sensitive and accurate and holds great potential for low-cost and high-throughput analysis of kynurenine in clinical laboratory settings.

Graphical abstract: Establishing a quantitative fluorescence assay for the rapid detection of kynurenine in urine

Supplementary files

Article information

Article type
Paper
Submitted
17 Jan 2022
Accepted
30 Mar 2022
First published
30 Mar 2022
This article is Open Access
Creative Commons BY license

Analyst, 2022,147, 1931-1936

Establishing a quantitative fluorescence assay for the rapid detection of kynurenine in urine

K. Patel, M. Fernandez-Villamarin, C. Ward, J. M. Lord, P. Tino and P. M. Mendes, Analyst, 2022, 147, 1931 DOI: 10.1039/D2AN00107A

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