Issue 43, 2005

Pathogen detection using single mode planar optical waveguides

Abstract

We utilize an optical waveguide-based biosensor recently developed at Los Alamos National Laboratory for the sensitive and specific detection of protein markers. Our planar optical waveguides are based on single mode structures that provide high optical field intensity at the active surface while providing discrimination from sample background fluorescence through spatial filtering. We have incorporated sandwich immunoassays where the capture antibody is immobilized on a biocompatible film attached to the surface of an optical waveguide. The approach can be adapted to any marker protein and is faster (<10 min), more sensitive, and as specific as conventional enzyme-linked immunosorbent assays (ELISA, the current lab standard). Results are presented for the detection of several analytes of Bacillus anthracis including protective antigen, a virulence marker for B. anthracis, and the bacterial cell or cellular debris.

Graphical abstract: Pathogen detection using single mode planar optical waveguides

Article information

Article type
Paper
Submitted
15 Feb 2005
Accepted
18 Aug 2005
First published
23 Sep 2005

J. Mater. Chem., 2005,15, 4639-4647

Pathogen detection using single mode planar optical waveguides

J. S. Martinez, W. K. Grace, K. M. Grace, N. Hartman and B. I. Swanson, J. Mater. Chem., 2005, 15, 4639 DOI: 10.1039/B502329G

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