Bio-sensing on a chip with compact discs and nanofibers

Abstract

This paper describes a novel, sensitive detection system for biomolecules (DNA and proteins etc.) that is integrated in a lab-on-a-chip utilizing optical compact discs (CDs) and bio-nanofibers. The new method comprises a microchannel containing CD grating that confines fragments of unique bacterial cellulose fibrils (BC), which have nanometre scale fibers and holes. A maximum of six times higher sensitivity to detect DNA was obtained with this CD and BC system compared to a conventional method. We also demonstrate an effective light-confining effect for biological application with the new method.