Issue 5, 2006

The living test-tube: imaging of real-time gene expression

Abstract

Cells are dynamic entities. Not only are some cells motile but there is constant motion of organelles, proteins, nucleic acids and other molecules within every living cell. These complex molecular pathways control the life cycle of a cell and all come down to the basic players of the gene expression pathway: DNA, RNA and protein. It is therefore imperative to study biological processes as they naturally occur—in living cells, and to unravel the biophysical rules that govern intracellular dynamics. Towards this end, genetically encoded fluorescent proteins have become one of the major tools available for the study of kinetic processes taking place in real-time. This review will focus on the technical developments available for the study of gene activity in living cells and will summarize the novel biological information extracted from these approaches.

Graphical abstract: The living test-tube: imaging of real-time gene expression

Article information

Article type
Review Article
Submitted
09 Jan 2006
Accepted
07 Feb 2006
First published
28 Feb 2006

Soft Matter, 2006,2, 361-370

The living test-tube: imaging of real-time gene expression

Y. Shav-Tal, Soft Matter, 2006, 2, 361 DOI: 10.1039/B600234J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements