Issue 45, 2007

Interrogation of living cells using alternating current scanning electrochemical microscopy (AC-SECM)

Abstract

In this paper we present the application of alternating current scanning electrochemical microscopy (AC-SECM) to the study of living cells. Commercial AFM instrumentation was modified to allow for performing robust AC-SECM measurements. Constant height AC imaging of the Cos-7 cells, performed directly in cell culture medium without the addition of a redox mediator, provided topographical information of the cell. Stationary tip measurements on the AC current were carried out to investigate the cellular activity of a single cell. The dependence of AC current magnitude on tip-to-sample separation distance was used to monitor real time changes in cell height of individual Cos-7 cells. Furthermore, AC-SECM was employed to observe changes in metabolic cellular activity stimulated by ethanol and phorbol-1,2-myristate-acetate-3. The effect of changing cellular activity on constant height AC-SECM imaging was also studied.

Graphical abstract: Interrogation of living cells using alternating current scanning electrochemical microscopy (AC-SECM)

Supplementary files

Article information

Article type
Paper
Submitted
26 Jul 2007
Accepted
24 Sep 2007
First published
09 Oct 2007

Phys. Chem. Chem. Phys., 2007,9, 5966-5974

Interrogation of living cells using alternating current scanning electrochemical microscopy (AC-SECM)

P. M. Diakowski and Z. Ding, Phys. Chem. Chem. Phys., 2007, 9, 5966 DOI: 10.1039/B711448F

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