Issue 6, 2008

Immunoassay for P38 MAPK using surface enhanced resonance Raman spectroscopy (SERRS)

Abstract

A micro-bead sandwich assay for P38 mitogen-activated protein kinase using surface enhanced resonance Raman spectroscopy (SERRS) detection is reported. Monoclonal capture antibodies were immobilised on a solid phase of magnetic micro-beads with secondary detection using a rhodamine-labelled antibody. Quantitative SERRS detection of the secondary antibody was possible with a limit of detection of 9.5 × 10−12 mol dm−3. The sandwich assay was quantitative and sensitive to 6 ng ml−1. The mechanism of the SERRS detection in the immunoassay was investigated. The addition of SERRS aggregating agents causes the dissociation of the immuno-complex from the magnetic beads. Scanning electron microscopy images indicate that the colloidal suspension rather than adsorbed silver nanoparticles on the beads provide the SERRS signals, that the aggregate size is partially controlled and that there is some inhomogeneity in the distribution of organic matter on the nanoscale.

Graphical abstract: Immunoassay for P38 MAPK using surface enhanced resonance Raman spectroscopy (SERRS)

Article information

Article type
Paper
Submitted
15 Oct 2007
Accepted
10 Mar 2008
First published
04 Apr 2008

Analyst, 2008,133, 791-796

Immunoassay for P38 MAPK using surface enhanced resonance Raman spectroscopy (SERRS)

P. Douglas, R. J. Stokes, D. Graham and W. E. Smith, Analyst, 2008, 133, 791 DOI: 10.1039/B715824F

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