Issue 5, 2008

Fabrication of a modular tissue construct in a microfluidic chip

Abstract

By combining microfluidics and soft-lithographic molding of gels containing mammalian cells, a device for three-dimensional (3D) culture of mammalian cells in microchannels was developed. Native components of the extracellular matrix, including collagen or Matrigel™, made up the matrix of each molded piece (module) of cell-containing gel. Each module had at least one dimension below ∼300 μm; in modules of these sizes, the flux of oxygen, nutrients, and metabolic products into and out of the modules was sufficient to allow cells in the modules to proliferate to densities comparable to those of native tissue (108–109cells cm−3). Packing modules loosely into microfluidic channels and chambers yielded structures permeated with a network of pores through which cell culture medium could flow to feed the encapsulated cells. The order in the packed assemblies increased as the width of the microchannels approached the width of the modules. Multiple cell types could be spatially organized in the small microfluidic channels. Recovery and analysis of modules after 24 h under constant flow of medium (200 μL h−1) showed that over 99% of encapsulated cells survived this interval in the microfluidic chamber.

Graphical abstract: Fabrication of a modular tissue construct in a microfluidic chip

Supplementary files

Article information

Article type
Paper
Submitted
04 Jan 2008
Accepted
05 Mar 2008
First published
20 Mar 2008

Lab Chip, 2008,8, 663-671

Fabrication of a modular tissue construct in a microfluidic chip

D. A. Bruzewicz, A. P. McGuigan and G. M. Whitesides, Lab Chip, 2008, 8, 663 DOI: 10.1039/B719806J

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