Issue 8, 2008

On-site manipulation of single chromosomal DNA molecules by using optically driven microstructures

Abstract

We report a novel method for manipulation of single giant DNA molecules under a video microscope. Using optically driven microstructures, we manipulated chromosomal DNA of length in the order of millimetres, extended by electroosmotic flow without DNA breakage in aqueous solution: we picked up DNA, using microfabricated hooks and wound it around microfabricated bobbins.

Graphical abstract: On-site manipulation of single chromosomal DNA molecules by using optically driven microstructures

Supplementary files

Article information

Article type
Paper
Submitted
06 Mar 2008
Accepted
19 May 2008
First published
23 Jun 2008

Lab Chip, 2008,8, 1280-1284

On-site manipulation of single chromosomal DNA molecules by using optically driven microstructures

K. Terao, M. Washizu and H. Oana, Lab Chip, 2008, 8, 1280 DOI: 10.1039/B803753A

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