Issue 9, 2008

Crossing microfluidic streamlines to lyse, label and wash cells

Abstract

We present a versatile method for continuous-flow, on-chip biological processing of cells, large bio-particles, and functional beads. Using an asymmetric post array in pressure-driven microfluidic flow, we can move particles of interest across multiple, independent chemical streams, enabling sequential chemical operations. With this method, we demonstrate on-chip cell treatments such as labeling and washing, and bacterial lysis and chromosomal extraction. The washing capabilities of this method are particularly valuable because they allow many analytical or treatment procedures to be cascaded on a single device while still effectively isolating their reagents from cross-contamination.

Graphical abstract: Crossing microfluidic streamlines to lyse, label and wash cells

Supplementary files

Article information

Article type
Paper
Submitted
15 Apr 2008
Accepted
02 Jul 2008
First published
23 Jul 2008

Lab Chip, 2008,8, 1448-1453

Crossing microfluidic streamlines to lyse, label and wash cells

K. J. Morton, K. Loutherback, D. W. Inglis, O. K. Tsui, J. C. Sturm, S. Y. Chou and R. H. Austin, Lab Chip, 2008, 8, 1448 DOI: 10.1039/B805614E

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