Issue 43, 2008

Greatly reduced amino acid alphabets in directed evolution: making the right choice for saturation mutagenesis at homologous enzyme positions

Abstract

Enantioselective mutants of the thermally robust phenyl acetone monooxygenase (PAMO) as catalysts in Baeyer–Villiger reactions have been evolved by utilizing saturation mutagenesis in which drastically reduced amino acid alphabets are employed at homologous enzyme positions.

Graphical abstract: Greatly reduced amino acid alphabets in directed evolution: making the right choice for saturation mutagenesis at homologous enzyme positions

Supplementary files

Article information

Article type
Communication
Submitted
01 Aug 2008
Accepted
12 Sep 2008
First published
02 Oct 2008

Chem. Commun., 2008, 5499-5501

Greatly reduced amino acid alphabets in directed evolution: making the right choice for saturation mutagenesis at homologous enzyme positions

M. T. Reetz and S. Wu, Chem. Commun., 2008, 5499 DOI: 10.1039/B813388C

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