Issue 20, 2009
From the journal:

Chemical Communications

Quantitation of biomolecules conjugated to nanoparticles by enzyme hydrolysis†

Fiona McKenzie,‡a   Victoria Steven,‡a   Andrew Ingrama  and  Duncan Graham*a  

* Corresponding authors

a Centre for Molecular Nanometrology, WestCHEM, Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow, UK
E-mail: duncan.graham@strath.ac.uk
Tel: +44 (0)141 548 4701

Abstract

A novel method for quantifying biomolecules immobilised onto gold and silver nanoparticles is reported; fluorescent-labelled antibodies and DNA are hydrolysed on the surface of the nanoparticles by the addition of trypsin and DNase I, respectively, resulting in the release of the quantifiable fluorescent label into the bulk solution.

Graphical abstract: Quantitation of biomolecules conjugated to nanoparticles by enzyme hydrolysis

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Article information

DOI
https://doi.org/10.1039/B823057A
Article type
Communication
Submitted
23 Dec 2008
Accepted
16 Mar 2009
First published
31 Mar 2009

Chem. Commun., 2009, 2872-2874

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Quantitation of biomolecules conjugated to nanoparticles by enzyme hydrolysis

F. McKenzie, V. Steven, A. Ingram and D. Graham, Chem. Commun., 2009, 2872 DOI: 10.1039/B823057A

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