Issue 18, 2009

Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme

Abstract

A chimeric oxygenase, in which the P450cam domain was fused to the reductase host domains of a P450RhF from Rhodococcus sp. strain NCIMB 9784 was optimised to allow for a biotransformation at 30 mM substrate in 80% overall yield, with the linker region between P450 and FMN domain proving to be important for the effective biotransformation of (+)-camphor to 5-exo-hydroxycamphor.

Graphical abstract: Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme

Supplementary files

Article information

Article type
Communication
Submitted
27 Jan 2009
Accepted
27 Feb 2009
First published
24 Mar 2009

Chem. Commun., 2009, 2478-2480

Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme

A. Robin, G. A. Roberts, J. Kisch, F. Sabbadin, G. Grogan, N. Bruce, N. J. Turner and S. L. Flitsch, Chem. Commun., 2009, 2478 DOI: 10.1039/B901716J

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