Issue 33, 2009

Catalytic properties of catechol 1,2-dioxygenase from Acinetobacter radioresistens S13 immobilized on nanosponges

Abstract

Catechol 1,2-dioxygenases are iron containing enzymes able to convert catechol into cis,cis-muconate, a precursor of the industrially important compound adipic acid. Catechol 1,2-dioxygenase from Acinetobacter radioresistens S13 was immobilized on β-cyclodextrins cross-linked with carbonate groups (nanosponges) with a yield of 29 mg of enzyme per gram of support. This support was chosen for its low cost and its ability to offer different types of interactions with the enzyme. The activity profiles at different pH and temperatures showed a shift of the optimal pH from 8.5, for the free protein, to 9.5, for the immobilized protein and, similarly, a shift in optimal temperature from 30 °C to 50 °C. The Michaelis–Menten constant, KM, increased from 2.0 ± 0.3 μM, for the free form, to 16.6 ± 4.8 μM for the immobilized enzyme, whereas the rate constant, kcat, values were found to be 32 ± 2 s−1 and 27 ± 3 s−1 for the free and immobilized forms respectively. The immobilization process also increased the thermostability of the enzyme with 60% residual activity after 90 min at 40 °C for the immobilized proteinversus 20% for the free enzyme. A residual activity of 75% was found after 15 min at 60 °C for the immobilized enzyme while the free form showed a total loss of activity under the same conditions. The activity toward other substrates, such as 3- and 4-methylcatechol and 4-chlorocatechol, was retained by the immobilized enzyme. A small scale bioreactor was constructed and was able to convert catechol into cis,cis-muconic acid with high efficiency for 70 days.

Graphical abstract: Catalytic properties of catechol 1,2-dioxygenase from Acinetobacter radioresistens S13 immobilized on nanosponges

Article information

Article type
Paper
Submitted
16 Feb 2009
Accepted
20 May 2009
First published
14 Jul 2009

Dalton Trans., 2009, 6507-6512

Catalytic properties of catechol 1,2-dioxygenase from Acinetobacter radioresistens S13 immobilized on nanosponges

G. Di Nardo, C. Roggero, S. Campolongo, F. Valetti, F. Trotta and G. Gilardi, Dalton Trans., 2009, 6507 DOI: 10.1039/B903105G

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