Issue 16, 2009

Transport of live yeast and zebrafish embryo on a droplet (“digital”) microfluidic platform

Abstract

We demonstrate the first programmed transport of live yeast (Saccharomyces cerevisiae) and a zebrafish embryo (Danio rerio) within droplets in a two-plate digital microfluidic device. The yeast remained viable after transport, and the actuated droplets left no yeast behind. A zebrafish embryo transported 2 hours after fertilization developed normally and hatched. Dechorionation was demonstrated by mixing a droplet of digestive reagent droplet with a droplet containing the embryo. These results demonstrate the potential for using a droplet microfluidic device as an alternative to microwell plates for yeast and zebrafish assays.

Graphical abstract: Transport of live yeast and zebrafish embryo on a droplet (“digital”) microfluidic platform

Supplementary files

Article information

Article type
Technical Note
Submitted
30 Mar 2009
Accepted
14 May 2009
First published
05 Jun 2009

Lab Chip, 2009,9, 2398-2401

Transport of live yeast and zebrafish embryo on a droplet (“digital”) microfluidic platform

S. U. Son and R. L. Garrell, Lab Chip, 2009, 9, 2398 DOI: 10.1039/B906257B

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