Issue 23, 2009
From the journal:

Organic & Biomolecular Chemistry

Synthesis of new, UV-photoactive dansyl derivatives for flow cytometric studies on bile acid uptake†

Jana Rohacova,a   M. Luisa Marin,a   Alicia Martínez-Romero,b   José-Enrique O'Connor,b   M. Jose Gomez-Lechon,cd   M. Teresa Donato,cde   Jose V. Castellcde  and  Miguel A. Miranda*a  

* Corresponding authors

a Instituto de Tecnología Química-Departamento de Química (UPV-CSIC), Avda de los Naranjos s/n, Valencia, Spain
E-mail: mmiranda@qim.upv.es
Fax: +34 963877809
Tel: +34 963877807

b Laboratorio de Citómica, Unidad Mixta CIPF-UVEG, Centro de Investigación Príncipe Felipe, Valencia, Spain

c Unidad de Hepatología Experimental, Centro de Investigación Hospital Universitario “La Fe”, Valencia, Spain

d CIBERHEPAD, FIS, Spain

e Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Valencia, Spain

Abstract

Four new fluorescent derivatives of cholic acid have been synthesized; they incorporate a dansyl moiety at 3α-, 3β-, 7α- or 7β- positions. These cholic acid analogs are UV photoactive and also exhibit green fluorescence. In addition, they have been demonstrated to be suitable for studying the kinetics of bile acid transport by flow cytometry.

Graphical abstract: Synthesis of new, UV-photoactive dansyl derivatives for flow cytometric studies on bile acid uptake

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Article information

DOI
https://doi.org/10.1039/B912134J
Article type
Paper
Submitted
25 Jun 2009
Accepted
26 Aug 2009
First published
24 Sep 2009

Org. Biomol. Chem., 2009,7, 4973-4980

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Synthesis of new, UV-photoactive dansyl derivatives for flow cytometric studies on bile acid uptake

J. Rohacova, M. L. Marin, A. Martínez-Romero, J. O'Connor, M. J. Gomez-Lechon, M. T. Donato, J. V. Castell and M. A. Miranda, Org. Biomol. Chem., 2009, 7, 4973 DOI: 10.1039/B912134J

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