Issue 10, 2010
From the journal:

Organic & Biomolecular Chemistry

Detection of pathogenic Streptococcus suis bacteria using magnetic glycoparticles†

Núria Parera Pera,‡a   Annika Kouki,‡b   Sauli Haataja,b   Hilbert M. Branderhorst,a   Rob M. J. Liskamp,a   Gerben M. Visser,c   Jukka Finned  and  Roland J. Pieters*a  

* Corresponding authors

a Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, P.O. Box 80082, The Netherlands
E-mail: R.J.Pieters@uu.nl
Fax: +31-30-2536655
Tel: +31-30-253944

b Department of Medical Biochemistry and Genetics, University of Turku, Finland

c Laboratory of Organic Chemistry, Wageningen University, Dreijenplein 8, The Netherlands

d Department of Biosciences, University of Helsinki, Finland

Abstract

Detection of the zoonotic bacterial pathogen Streptococcus suis was achieved using magnetic glycoparticles. The bacteria contain an adhesion protein for the carbohydrate sequence Galα1,4Gal. After incubation with various amounts of the pathogen, magnetic concentration and ATP detection, bacterial levels down to 105 cfu could be detected. Submicrometer particles were needed, since with the larger microparticles the method did not succeed.

Graphical abstract: Detection of pathogenic Streptococcus suis bacteria using magnetic glycoparticles

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C000819B
Article type
Paper
Submitted
15 Jan 2010
Accepted
08 Mar 2010
First published
22 Mar 2010

Org. Biomol. Chem., 2010,8, 2425-2429

Permissions

Request permissions

Detection of pathogenic Streptococcus suis bacteria using magnetic glycoparticles

N. P. Pera, A. Kouki, S. Haataja, H. M. Branderhorst, R. M. J. Liskamp, G. M. Visser, J. Finne and R. J. Pieters, Org. Biomol. Chem., 2010, 8, 2425 DOI: 10.1039/C000819B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements