Issue 43, 2010
From the journal:

Chemical Communications

Simultaneous detection of attomolar pathogen DNAs by Bio-MassCode mass spectrometry†

Bing Yang,‡a   Kai Gu,‡b   Xiaoru Sun,a   Huan Huang,c   Yibing Ding,a   Fuqiang Wang,d   Guohua Zhou*ac  and  Lequn Lee Huang*a  

* Corresponding authors

a Institute of Molecular Medicine and School of Medicine, Nanjing University, Nanjing 210093, China
E-mail: huanglq@nju.edu.cn, ghzhou@nju.edu.cn

b School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China

c Laboratory of Molecular Diagnostics, Huadong Research Institute for Medicine and Biotechnics, Nanjing 210002, China

d Nanjing Medical University, Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing, China

Abstract

A general method is described to analyze pathogen DNAs in homogenous solution by a novel Bio-MassCode probe, using a small organic (namely disulfide) “reporter mass” that self-assembled in oligonucleotide-modified gold nanoparticles. With conventional MALDI TOF MS, the assay simultaneously detected DNAs for HIV, HBV, HCV, and TP with extraordinarily high sensitivity in the low attomolar (10−18 M) range without any other amplification.

Graphical abstract: Simultaneous detection of attomolar pathogen DNAs by Bio-MassCode mass spectrometry

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Article information

DOI
https://doi.org/10.1039/C0CC03156A
Article type
Communication
Submitted
10 Aug 2010
Accepted
09 Sep 2010
First published
30 Sep 2010

Chem. Commun., 2010,46, 8288-8290

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Simultaneous detection of attomolar pathogen DNAs by Bio-MassCode mass spectrometry

B. Yang, K. Gu, X. Sun, H. Huang, Y. Ding, F. Wang, G. Zhou and L. L. Huang, Chem. Commun., 2010, 46, 8288 DOI: 10.1039/C0CC03156A

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