Issue 12, 2011

Dynamic interaction between melamine and cyanuric acid in artificial urine investigated by quartz crystal microbalance

Abstract

The dynamic interaction between melamine (M) and cyanuric acid (CA) in artificial urine was investigated by a flow injection analysis-quartz crystal microbalance (FIA-QCM) system. Melamine was used as the recognition element and immobilized onto the QCM gold surface. The process of M and CA interaction was recorded by FIA-QCM in real-time. The multilayer complex of M and CA was successfully formed on the crystal surface of the QCM when CA and M were introduced into the FIA-QCM system alternately. The influence of pH on the M and CA interaction indicated that the M–CA multilayer complex possesses high stability over a wide range of pH values, especially in physiological urine condition. The association and dissociation rate constants were determined under artificial urine condition and the association constant for M and CA was calculated as 102 L mol−1. The M–CA complex was further characterized by Fourier transform infrared spectroscopy (FT-IR) and atomic force microscopy (AFM). Mechanism study suggested that the formation of the multilayer complex in artificial urine was mainly attributed to the intermolecular hydrogen bonding between M and CA. The work provides direct insight into the dynamic interaction between M and CA under physiological conditions and would be helpful for the research of kidney stones induced by melamine contamination.

Graphical abstract: Dynamic interaction between melamine and cyanuric acid in artificial urine investigated by quartz crystal microbalance

Supplementary files

Article information

Article type
Paper
Submitted
13 Feb 2011
Accepted
25 Apr 2011
First published
11 May 2011

Analyst, 2011,136, 2482-2488

Dynamic interaction between melamine and cyanuric acid in artificial urine investigated by quartz crystal microbalance

Y. Xie, Y. Huang, W. Wang, G. Liu and R. Zhao, Analyst, 2011, 136, 2482 DOI: 10.1039/C1AN15119C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements