Issue 8, 2011

Amplified ribosomal DNA restriction analysis as a routine tool to assess toxicant driven changes in hindgut bacterial populations of Porcellio dilatatus (Crustacea: Isopoda)

Abstract

Changes in saprophytic organism's gut microbial communities may present a threat to organic matter breakdown which can ultimately lead to soil function impairment. In this study, Amplified Ribosomal DNA Restriction Analysis (ARDRA) was evaluated as a potential simple molecular tool to assess shifts in bacterial community structure in hindgut populations of Porcellio dilatatus exposed to contaminated food. This prospective tool can also be used for a variety of purposes and samples prior to the use of more specific and sophisticated methods.

Graphical abstract: Amplified ribosomal DNA restriction analysis as a routine tool to assess toxicant driven changes in hindgut bacterial populations of Porcellio dilatatus (Crustacea: Isopoda)

Supplementary files

Article information

Article type
Communication
Submitted
11 Feb 2011
Accepted
08 Jun 2011
First published
15 Jul 2011

J. Environ. Monit., 2011,13, 2102-2104

Amplified ribosomal DNA restriction analysis as a routine tool to assess toxicant driven changes in hindgut bacterial populations of Porcellio dilatatus (Crustacea: Isopoda)

M. J. G. Santos, J. P. Sousa, I. Tiago, A. Veríssimo and M. F. L. Lemos, J. Environ. Monit., 2011, 13, 2102 DOI: 10.1039/C1EM10137D

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