Issue 52, 2012
From the journal:

Chemical Communications

A selective Seoul-Fluor-based bioprobe, SfBP, for vaccinia H1-related phosphatase—a dual-specific proteintyrosine phosphatase†

Myeong Seon Jeong,‡a   Eunha Kim,‡b   Hyo Jin Kang,a   Eun Joung Choi,b   Alvin R. Cho,§b   Sang J. Chung*a  and  Seung Bum Park*bc  

* Corresponding authors

a BioNanotechnology Research Center, KRIBB and NanoBio Major, UST, 111 Kwahangno, Yuseong, Daejeon 305-806, Korea
E-mail: sjchung@kribb.re.kr
Fax: +82 42 879 8594
Tel: +82 42 879 8433

b Department of Chemistry, Seoul National University, Seoul, Korea

c Department of Biophysics and Chemical Biology/BioMAX Institute, Seoul National University, Seoul 151-747, Korea
E-mail: sbpark@snu.ac.kr
Fax: +82 2 884 4025
Tel: +82 2 880 9090

Abstract

We report a Seoul-Fluor-based bioprobe, SfBP, for selective monitoring of protein tyrosine phosphatases (PTPs). A rational design based on the structures at the active site of dual-specific PTPs can enable SfBP to selectively monitor the activity of these PTPs with a 93-fold change in brightness. Moreover, screening results of SfBP against 30 classical PTPs and 35 dual-specific PTPs show that it is selective toward vaccinia H1-related (VHR) phosphatase, a dual-specific PTP (DUSP-3).

Graphical abstract: A selective Seoul-Fluor-based bioprobe, SfBP, for vaccinia H1-related phosphatase—a dual-specific protein tyrosine phosphatase

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Article information

DOI
https://doi.org/10.1039/C2CC31377D
Article type
Communication
Submitted
07 Jan 2012
Accepted
04 May 2012
First published
04 May 2012

Chem. Commun., 2012,48, 6553-6555

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A selective Seoul-Fluor-based bioprobe, SfBP, for vaccinia H1-related phosphatase—a dual-specific protein tyrosine phosphatase

M. S. Jeong, E. Kim, H. J. Kang, E. J. Choi, A. R. Cho, S. J. Chung and S. B. Park, Chem. Commun., 2012, 48, 6553 DOI: 10.1039/C2CC31377D

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