Issue 72, 2015

Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd3+-DOTA

Abstract

Gd3+ is increasingly used in EPR spectroscopy due to its increased intracellular stability and signal-to-noise ratios. Here we present the incorporation of Gd3+-DOTA into internal positions in DNA. Distance measurements via pulsed Electron Paramagnetic Resonance (EPR) spectroscopy in vitro and in cellula proved enhanced stability and efficiency compared to nitroxide labels.

Graphical abstract: Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd3+-DOTA

Supplementary files

Article information

Article type
Communication
Submitted
22 May 2015
Accepted
22 Jul 2015
First published
22 Jul 2015
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2015,51, 13850-13853

Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd3+-DOTA

F. Wojciechowski, A. Groß, I. T. Holder, L. Knörr, M. Drescher and J. S. Hartig, Chem. Commun., 2015, 51, 13850 DOI: 10.1039/C5CC04234H

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements