Issue 5, 2016

Molecular basis of 5-hydroxytryptophan synthesis in Saccharomyces cerevisiae

Abstract

We report for the first time that 5-hydroxytryptophan can be synthesized in Saccharomyces cerevisiae by heterologously expressing prokaryotic phenylalanine 4-hydroxylase or eukaryotic tryptophan 3/5-hydroxylase, together with enhanced synthesis of MH4 or BH4 cofactors. The innate DFR1 gene in the folate synthesis pathway was found to play pivotal roles in 5-hydroxytryptophan synthesis.

Graphical abstract: Molecular basis of 5-hydroxytryptophan synthesis in Saccharomyces cerevisiae

Supplementary files

Article information

Article type
Communication
Submitted
18 Dec 2015
Accepted
18 Mar 2016
First published
18 Mar 2016
This article is Open Access
Creative Commons BY-NC license

Mol. BioSyst., 2016,12, 1432-1435

Molecular basis of 5-hydroxytryptophan synthesis in Saccharomyces cerevisiae

J. Zhang, C. Wu, J. Sheng and X. Feng, Mol. BioSyst., 2016, 12, 1432 DOI: 10.1039/C5MB00888C

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