Issue 7, 2016

Real time monitoring of aminothiol level in blood using a near-infrared dye assisted deep tissue fluorescence and photoacoustic bimodal imaging

Abstract

The development of molecular probes for the detection and imaging of biological thiols is a major step forward diagnosing various types of diseases. Previously reported thiol imaging strategies were mainly based on a single mode of imaging with a limited application in vivo. In this work, we introduced an unsymmetrical near-infrared (NIR) squaraine dye (USq) as an exogenous contrast agent for photoacoustic and fluorescence bimodal imaging of thiol variations in live animals. USq exhibits a narrow absorption band at 680 nm that generates a photoacoustic signal and a strong NIR emission at 700 nm (ΦF = 0.27), which is applicable for deep tissue optical imaging. Both photoacoustic and fluorescence signals could selectively disappear in the presence of different thiols. Through in vitro and in vivo imaging studies, unique imaging capability of USq was demonstrated, and the effect of food uptake on the increased level of aminothiols in blood was confirmed.

Graphical abstract: Real time monitoring of aminothiol level in blood using a near-infrared dye assisted deep tissue fluorescence and photoacoustic bimodal imaging

Supplementary files

Article information

Article type
Edge Article
Submitted
29 Dec 2015
Accepted
02 Mar 2016
First published
02 Mar 2016
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2016,7, 4110-4116

Real time monitoring of aminothiol level in blood using a near-infrared dye assisted deep tissue fluorescence and photoacoustic bimodal imaging

P. Anees, J. Joseph, S. Sreejith, N. V. Menon, Y. Kang, S. Wing-Kwong Yu, A. Ajayaghosh and Y. Zhao, Chem. Sci., 2016, 7, 4110 DOI: 10.1039/C5SC04986E

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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