Enzymatic synthesis and reverse transcription of RNAs incorporating 2′-O-carbamoyl uridine triphosphate

Yoshiaki Masaki; Hyugo Ito; Yuki Oda; Kazufumi Yamazaki; Nobuhiro Tago; Kentaro Ohno; Nozomi Ishii; Hirosuke Tsunoda; Takashi Kanamori; Akihiro Ohkubo; Mitsuo Sekine; Kohji Seio

doi:10.1039/C6CC05796A

Enzymatic synthesis and reverse transcription of RNAs incorporating 2′-O-carbamoyl uridine triphosphate†

Yoshiaki Masaki,^a Hyugo Ito,^a Yuki Oda,^a Kazufumi Yamazaki,^a Nobuhiro Tago,^a Kentaro Ohno,^a Nozomi Ishii,^a Hirosuke Tsunoda,^a Takashi Kanamori,^a Akihiro Ohkubo,^a Mitsuo Sekine^a and Kohji Seio*^a

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^a Department of Life Science and Technology, Tokyo Institute of Technology, J2-16, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Japan
E-mail: kseio@bio.titech.ac.jp

Abstract

Enzymatic synthesis and the reverse transcription of RNAs containing 2′-O-carbamoyl uridine were evaluated. A mild acidic deprotection procedure allowed the synthesis of 2′-O-carbamoyl uridine triphosphate (U_cmTP). U_cmTP was incorporated correctly into long RNAs, and its fidelity during reverse transcription using SuperScript III was sufficient for RNA aptamer selection.

Chemical Communications

Enzymatic synthesis and reverse transcription of RNAs incorporating 2′-O-carbamoyl uridine triphosphate†

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Enzymatic synthesis and reverse transcription of RNAs incorporating 2′-O-carbamoyl uridine triphosphate

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