Issue 17, 2017

Imidazolium-labeled glycosides as probes to harness glycosyltransferase activity in human breast milk

Abstract

Imidazolium-labeled (ITag-) glycosides are used to harness the glycosyltransferase activity directly from human breast milk. The covalently attached ionic labels provide a bifunctional chemical handle that is used to monitor reaction progress by MS, as well as aid in product purification from complex mixtures. The technology is exemplified in the synthesis of biologically relevant oligosaccharide analogs, LacNAc-ITag, ITag-Lewisx and ITag-Lewisa, in a matter of days from human breast milk without having to isolate specific enzymes.

Graphical abstract: Imidazolium-labeled glycosides as probes to harness glycosyltransferase activity in human breast milk

Supplementary files

Article information

Article type
Communication
Submitted
03 Mar 2017
Accepted
06 Apr 2017
First published
06 Apr 2017
This article is Open Access
Creative Commons BY license

Org. Biomol. Chem., 2017,15, 3575-3579

Imidazolium-labeled glycosides as probes to harness glycosyltransferase activity in human breast milk

I. Sittel and M. C. Galan, Org. Biomol. Chem., 2017, 15, 3575 DOI: 10.1039/C7OB00550D

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