Issue 48, 2017

A colorimetric competitive displacement assay for the evaluation of catalytic peptides

Abstract

An indicator displacement assay has been adapted to detect the diol products of the aldol reaction between 4-nitrobenzaldehyde and hydroxyacetone in crude reaction mixtures. This provides a rapid colorimetric method of detecting product formation and thus evaluating potential catalysts, which is demonstrated using multiple catalytic peptides.

Graphical abstract: A colorimetric competitive displacement assay for the evaluation of catalytic peptides

Supplementary files

Article information

Article type
Communication
Submitted
15 Aug 2017
Accepted
24 Oct 2017
First published
28 Nov 2017
This article is Open Access
Creative Commons BY-NC license

Org. Biomol. Chem., 2017,15, 10160-10163

A colorimetric competitive displacement assay for the evaluation of catalytic peptides

A. M. M. Gest, E. M. Aguiluz, M. T. Mays, X. Liu, E. K. Neidhart and L. S. Witus, Org. Biomol. Chem., 2017, 15, 10160 DOI: 10.1039/C7OB02032E

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