Issue 72, 2017, Issue in Progress

Hydrophilic cell-derived extracellular matrix as a niche to promote adhesion and differentiation of neural progenitor cells

Abstract

The natural extracellular matrix (ECM) offers a dynamic and intricate microenvironment, which serves as a structural support and regulates cell phenotype and its function. Recently, ECM has been revealed as a favorable and biocompatible architecture for stem cell adhesion and growth in cellular therapy to treat various diseases. However, cell-derived ECM is rarely used as a culture substrate for anchorage-dependent cells, such as neural progenitor cells (NPCs), which have the potential to differentiate into basal forebrain cholinergic neurons (BFCNs) for Alzheimer's disease. Here, we report mouse embryonic fibroblast (MEF)-derived ECM, with an appropriately hydrophilic property (water contact angle of 66.8°) to mimic the neural niche for NPCs. In addition, MEF-derived ECM possesses a nanotopological surface, plentiful kinds of components and exhibits excellent adhesion properties for anchoring NPCs. Compared with a laminin-coated plate, MEF-derived ECM promotes NPC proliferation and differentiation into BFCNs by ∼1.6 fold and ∼3.1 fold, respectively, consequently enhancing the production of acetylcholine by ∼2.0 fold. This MEF-derived ECM could be a favorable cell culture carrier for NPC attachment, with great potential for applications in stem cell therapy for Alzheimer's disease.

Graphical abstract: Hydrophilic cell-derived extracellular matrix as a niche to promote adhesion and differentiation of neural progenitor cells

Supplementary files

Article information

Article type
Paper
Submitted
27 Jul 2017
Accepted
20 Sep 2017
First published
27 Sep 2017
This article is Open Access
Creative Commons BY license

RSC Adv., 2017,7, 45587-45594

Hydrophilic cell-derived extracellular matrix as a niche to promote adhesion and differentiation of neural progenitor cells

L. Yang, Z. Jiang, L. Zhou, K. Zhao, X. Ma and G. Cheng, RSC Adv., 2017, 7, 45587 DOI: 10.1039/C7RA08273H

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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