Issue 10, 2017

Reversible control of cell membrane receptor function using DNA nano-spring multivalent ligands

Abstract

Chemically functionalized and nanostructured materials, which mimic the features of the natural extracellular matrix, provide a tool to organize cell surface receptors into nanoscale clusters and manipulate cell functions. However, the existing materials are mainly based on static structures. Herein, we developed a DNA based structure-switchable and multivalent material that acts as a ‘nano-spring’, enabling reversible control of membrane receptor function at the cell surface. This ‘nano-spring’ can be easily synthesized by rolling circle amplification and finely tuned by changing the circular template design. Using this ‘nano-spring’ to interact with cells, we have demonstrated that the movement of the DNA nanostructure is sufficient to direct a cell morphology change from the normal morphology to having numerous cell protrusions and affect the mRNA expression level of integrin related genes. This DNA nano-spring structure can be a competitive material for actively manipulating cell receptor function and may help us to understand the role of receptor mediated signalling cascades.

Graphical abstract: Reversible control of cell membrane receptor function using DNA nano-spring multivalent ligands

Supplementary files

Article information

Article type
Edge Article
Submitted
03 Jun 2017
Accepted
17 Aug 2017
First published
18 Aug 2017
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2017,8, 7098-7105

Reversible control of cell membrane receptor function using DNA nano-spring multivalent ligands

K. Zhang, R. Deng, Y. Sun, L. Zhang and J. Li, Chem. Sci., 2017, 8, 7098 DOI: 10.1039/C7SC02489D

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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