Issue 36, 2018

Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures

Abstract

Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA that is compatible with polymerase enzymes.

Graphical abstract: Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures

Supplementary files

Article information

Article type
Communication
Submitted
28 Jan 2018
Accepted
20 Mar 2018
First published
17 Apr 2018
This article is Open Access
Creative Commons BY license

Chem. Commun., 2018,54, 4529-4532

Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures

I. Manuguerra, S. Croce, A. H. El-Sagheer, A. Krissanaprasit, T. Brown, K. V. Gothelf and A. Manetto, Chem. Commun., 2018, 54, 4529 DOI: 10.1039/C8CC00738A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements