Issue 8, 2019

High-throughput single-particle detections using a dual-height-channel-integrated pore

Abstract

We report a proof-of-principle demonstration of particle concentration to achieve high-throughput resistive pulse detections of bacteria using a microfluidic-channel-integrated micropore. We fabricated polymeric nanochannels to trap micrometer-sized bioparticles via a simple water pumping mechanism that allowed aggregation-free size-selective particle concentration with negligible loss. Single-bioparticle detections by ionic current measurements were then implemented through releasing and transporting the thus-collected analytes to the micropore. As a result, we attained two orders of magnitude enhancement in the detection throughput by virtue of an accumulation effect via hydrodynamic control. The device concept presented may be useful in developing nanopores and nanochannels for high-throughput single-particle and -molecule analyses.

Graphical abstract: High-throughput single-particle detections using a dual-height-channel-integrated pore

Supplementary files

Article information

Article type
Paper
Submitted
16 Dec 2018
Accepted
16 Feb 2019
First published
25 Mar 2019
This article is Open Access
Creative Commons BY-NC license

Lab Chip, 2019,19, 1352-1358

High-throughput single-particle detections using a dual-height-channel-integrated pore

W. Tonomura, M. Tsutsui, A. Arima, K. Yokota, M. Taniguchi, T. Washio and T. Kawai, Lab Chip, 2019, 19, 1352 DOI: 10.1039/C8LC01371C

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