Issue 46, 2018

The study of multivalent carbohydrate–protein interactions by bio-layer interferometry

Abstract

The study of complex multivalent carbohydrate–protein interactions remains highly complicated and sometimes rendered impossible due to aggregation problems. In this study, we demonstrate that bio-layer interferometry is an excellent complementary method to standard techniques such as SPR and ITC. Using tetra- and hexadecavalent GalNAc glycoconjugates and Helix pomatia agglutinin (HPA) as a model lectin, we were able to measure reliable kinetic and thermodynamic parameters of multivalent interactions going from the micro to the nanomolar range.

Graphical abstract: The study of multivalent carbohydrate–protein interactions by bio-layer interferometry

Supplementary files

Article information

Article type
Communication
Submitted
13 Jul 2018
Accepted
17 Aug 2018
First published
28 Sep 2018
This article is Open Access
Creative Commons BY-NC license

Org. Biomol. Chem., 2018,16, 8899-8903

The study of multivalent carbohydrate–protein interactions by bio-layer interferometry

E. Laigre, D. Goyard, C. Tiertant, J. Dejeu and O. Renaudet, Org. Biomol. Chem., 2018, 16, 8899 DOI: 10.1039/C8OB01664J

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements