Issue 49, 2018, Issue in Progress

Facile fabrication of a recyclable nanobiocatalyst: immobilization of Burkholderia cepacia lipase on carbon nanofibers for the kinetic resolution of a racemic atenolol intermediate

Abstract

Immobilization of surfactant treated Burkholderia cepacia lipase on the surface of carbon nanofibers was performed via two different methods: adsorption and covalent attachment. Simple adsorption of lipase on carbon nanofibers turned out to be a poor strategy, exhibiting an immobilization efficiency of 36%, while covalent coupling using 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide (EDC)/N-hydroxysuccinimide (NHS) showed better immobilization efficiency (56%). The nanobioconjugate fabricated using the latter method showed an eleven-fold increase in enzyme activity towards the hydrolysis of p-nitrophenyl palmitate and enhanced dispersion in organic solvents. At 80 °C, the half-life of lipase in the nanobioconjugate was almost 20 fold higher than that of free lipase, demonstrating its thermal stability. The as-prepared nanobioconjugate was reused for nine consecutive reaction cycles achieving 100% yield in the hydrolysis of p-nitrophenol palmitate but losing almost 50% of the initial activity after seven operational cycles. Finally, this heterogeneous nanobioconjugate was more active and enantioselective [C = 47.8, eep = 97.0 and E = 194] than free lipase [C = 35.4, eep = 97.1 and E = 88] towards the kinetic resolution of a racemic intermediate of atenolol yielding the S enantiomer, which signifies its importance as a nanobiocatalyst.

Graphical abstract: Facile fabrication of a recyclable nanobiocatalyst: immobilization of Burkholderia cepacia lipase on carbon nanofibers for the kinetic resolution of a racemic atenolol intermediate

Supplementary files

Article information

Article type
Paper
Submitted
26 Jun 2018
Accepted
25 Jul 2018
First published
03 Aug 2018
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2018,8, 27763-27774

Facile fabrication of a recyclable nanobiocatalyst: immobilization of Burkholderia cepacia lipase on carbon nanofibers for the kinetic resolution of a racemic atenolol intermediate

S. Soni, B. P. Dwivedee and U. Chand Banerjee, RSC Adv., 2018, 8, 27763 DOI: 10.1039/C8RA05463K

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