Issue 1, 2019

Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells

Abstract

We developed a fast strain-promoted azide–alkyne cycloaddition reaction (SPAAC) by tetra-fluorinated aromatic azide with a kinetic constant of 3.60 M−1 s−1, which is among the fastest SPAAC ligations reported so far. We successfully employed the reaction for covalent labelling of proteins with high efficiency and for bioimaging of mitochondria in living cells. The reaction could be a generally useful toolbox for chemical biology and biomaterials.

Graphical abstract: Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells

Supplementary files

Article information

Article type
Paper
Submitted
11 Nov 2018
Accepted
10 Dec 2018
First published
19 Dec 2018
This article is Open Access
Creative Commons BY license

RSC Adv., 2019,9, 23-26

Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells

X. Cai, D. Wang, Y. Gao, L. Yi, X. Yang and Z. Xi, RSC Adv., 2019, 9, 23 DOI: 10.1039/C8RA09303B

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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